Journal: Journal of proteome research
Article Title: Combined Antibody/Lectin-Enrichment Identifies Extensive Changes in the O -GlcNAc Sub-proteome Upon Oxidative Stress
doi: 10.1021/acs.jproteome.6b00369
Figure Lengend Snippet: O -GlcNAcylated peptides identified from proteins that were observed to be differentially associated with the G5-lectibody column by ≤0.7 or ≥1.3 ( Supplementary Information , Table 2 , Supplemental Spectra ).
Article Snippet: Not surprisingly, 32 peptides (with multiple O -GlcNAc sites) corresponded to the highly O -GlcNAcylated protein Host Cell Factor 1, which has been characterized to contain > 30 O -GlcNAcylation sites among different cells and tissue types 134 . table ft1 table-wrap mode="anchored" t5 Table 2 – caption a7 Accession (GI number) Protein Sequence Site Fold change (1 hr) Fold change (2 hr) 40549397 Ankyrin repeat domain-containing protein 17 1993 QLFV gT VVK 2000 T1997 1.37 1.08 2186 gT PAPVQSSSASVLNVNHIK 204 T2186 124244104 Ataxin-2 167 KPGGGLL SS PGAAPA S AAV TS ASVVPAPAAPVA SSS AAAGGGR 209 N.D. 1.39 1.27 210147541 CCR4-NOT transcription complex subunit 2 isoform a 58 S L S QG T QLP S HV T P TT GVP T M S LH T PP S P S R 88 N.D § 1.51 1.11 181332994 glutamine and serine-rich protein 72 TS QG T VP T ALAFER 85 N.D. § 0.81 0.32 208 D ST QV S NGVLPQK 220 N.D. § 254588102 Nucleoporin 153 618 VD S PALQP TT T S SIVY T R 635 N.D. Di-Glycopeptide § 0.67 0.72 1006 QQEPV TSTS LVFGK 1109 N.D. § 124378033 Nucleoporin 214 489 SSASVTGEPPLYPTGSDS gS R 508 S507 0.78 0.54 611 PTA S GPL S HP T PLPA SSSS MPLK 633 N.D. 1307 PAAPPE TT V SS A SS K 1321 N.D. 1550 EPVLVQ TT D SS P S R 1563 N.D. 124249084 Protein EMSY 498 TTS G S II T VVPK 510 N.D. 0.58 0.12 518 II SS NIVSG TTT K 530 N.D. § 255522770 serine/threonine-protein kinase ICK 445 FE S VLDLKP S E S VG T G TT V ST QA SS QR 472 N.D. N.D. 1.49 124517678 Tyrosine-protein phosphatase non-receptor type 23 878 PA TTT VD S VQAPI SS H T APR 897 N.D. 0.61 0.52 260166706 Ubiquitin-associated protein 2-like isoform 4 442 QAF T P SST MMEVFLQEKPPAVA TST AAPPPP SS PLP S K 479 N.D 1.31 1.03 Open in a separate window § Glycopeptide or glycosylation site previously identified and catalogued in dbOGAP or Cell Signaling Technology databases.
Techniques: Sequencing